Epigallocatechin gallate (EGCG) activity against UV light-induced photo damages in erythrocytes and serum albumin—theoretical and experimental studies

　

　

Highlights

• EGCG protects erythrocytes against UVB-induced damages.

 

• EGCG preventing lipids, glutathione and hemoglobin oxidation.

 

• EGCG decreases photo-damage in bovine serum albumin.

 

• EGCG can be useful in protection of blood components against damages evoked by UVB.

 

 

Abstract

Ultraviolet (UV) exerts significant toxic effects on such blood components as erythrocytes and serum albumin under the influence of sunlight, during phototherapy, photodynamic therapy and pathogen reduction technology. In this study, we showed that epigallocatechin gallate (EGCG) isolated from Geranium rectum roots at concentrations of 1–20 μM protected erythrocytes against UVB induced damages, preventing ROS (reactive oxygen species) formation, lipid peroxidation (LP), reduced glutathione (GSH) and hemoglobin (Hb) oxidation. Our data on fluorescence anisotropy measurements noted that EGCG significantly increased the fluidity of the outer leaflet layer of erythrocytes membrane and induced a stiffening in the nonpolar region. We demonstrated that UVB irradiation of bovine serum albumin (BSA) induced SS disruption and SH group formation, which was prevented by EGCG in a dose-dependent manner. The analysis of radical-flavonoid interaction’s stoichiometry in the EGCG-DPPH model revealed that one EGCG molecule was consumed for reduction of two DPPH molecules. On the basis of theoretical calculation it was shown that the hydroxyl groups in positions 4′ and 5′ of the ring B could be considered as the most preferable H-atoms donators. Thus, the theoretical and experimental data showed that EGCG can be useful in protection of blood components against oxidative damages induced by UVB.

　

Journal of Photochemistry and Photobiology A: Chemistry

Volume 356, 1 April 2018, Pages 379-388

　

Author links open overlay panelSzymonSekowskiaMilenaTerebkaaArtemVeikobElenaLapshinabUrszulaSulkowskaaIlya B.ZavodnikbNodiraAbdulladjanovacSaidmukhtarMavlyanovcAnnaRoszkowskaaMariaZamaraevaa

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https://doi.org/10.1016/j.jphotochem.2018.01.018Get rights and content

　

　

Epigallocatechin gallate (EGCG) activity against UV light-induced photo damages in erythrocytes and serum albumin—theoretical and experimental studies - ScienceDirect  https://www.sciencedirect.com/science/article/abs/pii/S101060301731403X