蓝光诱导活皮肤氧化应激 有助于皮肤老化 （同时证明蓝光有杀菌作用）
Blue light-induced oxidative stress in live skin
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要点
• 蓝光照射在活鼠皮肤和人角质细胞中氧化谷胱甘肽。
• 蓝色光子ROS的生产效率在小鼠为UVA的68%，在人类细胞为UVA的25%。
• 蓝光光敏剂是黄素，而ROS产生的是超氧化物。
• 天然阳光中的蓝色成分可以破坏人体皮肤中的黄素。
• 吸收短波蓝光的防晒霜会减少ROS的产生。
暴露在阳光下的皮肤损害会诱发衰老，这是由于光的紫外线(UV)成分造成的。UVA光对活性氧(ROS)的光敏化生产被广泛接受，导致皮肤损伤和癌变，但是可见光被认为不会导致皮肤损伤。
用小鼠表达氧化还原反应的GFP检测ROS，蓝光会在活皮肤中产生氧化应激。
蓝光诱导的氧化应激优先于线粒体，而绿色、红色、远红光或红外光则没有。
在人工培养的人角质细胞中也检测到蓝色光诱导的氧化应激，但在人角质细胞线粒体中，每个光子的功效仅为UVA的25%，而在小鼠皮肤中为UVA的68%。
用蓝光降低皮肤自身荧光，说明黄素是光敏剂。将人的皮肤暴露在阳光下的蓝光中抑制了黄酮的自发荧光，证明了阳光的可见成分对人的皮肤有生理上的显著影响。
蓝光产生的活性氧可能是超氧化物，而不是单态氧。这些结果表明蓝光有助于皮肤老化，类似于UVA。
Blue light-induced oxidative stress in live skin
Author links open overlay panelYuyaNakashimaShigeoOhtaAlexander M.Wolf
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https://doi.org/10.1016/j.freeradbiomed.2017.03.010Get rights and content
Highlights
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Blue light exposure oxidizes glutathione in live mouse skin and human keratinocytes.
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Blue photon ROS production efficacy is 68% of UVA in mice & 25% in human cells.
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The blue light photosensitizer is flavin and the ROS produced is superoxide.
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The blue component of natural sunlight could destroy flavins in live human skin.
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Suncreens absorbing short wavelength blue light would reduce ROS production.
Skin damage from exposure to sunlight induces aging-like changes in appearance and is attributed to the ultraviolet (UV) component of light. Photosensitized production of reactive oxygen species (ROS) by UVA light is widely accepted to contribute to skin damage and carcinogenesis, but visible light is thought not to do so. Using mice expressing redox-sensitive GFP to detect ROS, blue light could produce oxidative stress in live skin. Blue light induced oxidative stress preferentially in mitochondria, but green, red, far red or infrared light did not. Blue light-induced oxidative stress was also detected in cultured human keratinocytes, but the per photon efficacy was only 25% of UVA in human keratinocyte mitochondria, compared to 68% of UVA in mouse skin. Skin autofluorescence was reduced by blue light, suggesting flavins are the photosensitizer. Exposing human skin to the blue light contained in sunlight depressed flavin autofluorescence, demonstrating that the visible component of sunlight has a physiologically significant effect on human skin. The ROS produced by blue light is probably superoxide, but not singlet oxygen. These results suggest that blue light contributes to skin aging similar to UVA.
Blue light-induced oxidative stress in live skin - ScienceDirect https://www.sciencedirect.com/science/article/pii/S089158491730134X
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