芹菜素通过降解缺氧诱导因子HIF-1，抑制内皮细胞血管生成的重要因子VEGF的表达

Apigenin suppresses the expression of VEGF, an important factor for angiogenesis, in endothelial cells via degradation of HIF-1alpha protein

　

芹菜素是植物来源的黄酮，是细胞增殖和血管生成的有效抑制剂，但导致芹菜素的病理性抗血管生成作用的机制仍不清楚。在本研究中，我们发现芹菜素抑制缺氧诱导的人脐动脉内皮细胞血管内皮生长因子（VEGF）mRNA的表达。芹菜素还通过降解缺氧诱导因子1（HIF-1）α抑制促红细胞生成素mRNA的表达，促红细胞生成素mRNA是典型的缺氧诱导基因。我们研究了芹菜素对HIF-1α与热休克蛋白90（Hsp90）相互作用的影响，据报道热休克蛋白对HIF-1α的稳定具有重要作用。发现芹菜素通过干扰Hsp90的功能，降解HIF-1α可抑制VEGF的表达。。

 

Apigenin suppresses the expression of VEGF, an important factor for angiogenesis, in endothelial cells via degradation of HIF-1alpha protein

 

Apigenin, a plant-derived flavone, is a potent inhibitor of cell proliferation and angiogenesis, but the mechanisms leading to the pathological anti-angiogenic effects of apigenin are still unclear. In this study, we found that apigenin inhibited the hypoxia-induced expression of vascular endothelial growth factor (VEGF) mRNA in human umbilical artery endothelial cells. Apigenin also suppressed the expression of erythropoietin mRNA, which is a typical hypoxia-inducible gene, via the degradation of hypoxia-inducible factor 1 (HIF-1) alpha. We investigated the effect of apigenin on the interaction of HIF-1alpha with heat shock protein 90 (Hsp90), which is reported to be important for the stabilization of HIF-1alpha, and found that VEGF expression was inhibited via degradation of HIF-1alpha through interference with the function of Hsp90.

 

 

Apigenin suppresses the expression of VEGF, an important factor for angiogenesis, in endothelial cells via degradation of HIF-1?? protein. Available from: https://www.researchgate.net/publication/8327360_Apigenin_suppresses_the_expression_of_VEGF_an_important_factor_for_angiogenesis_in_endothelial_cells_via_degradation_of_HIF-1_protein [accessed Oct 30 2018].