脂肪酸对内皮细胞的影响:炎症和单核细胞粘附

Effects of fatty acids on endothelial cells: inflammation and monocyte adhesion

 

MarleneGrenonMDCMabcJesusAguado-ZunigaBSabJason PHattonPhDbChristopher DOwensMDacMichael S.ConteMDaMillieHughes-FulfordPhDbd

 

 

 

文摘

 

背景

众所周知,饮食对心血管健康有重要的影响。鱼油中含有大量的n-3脂肪酸(FAs),在冠心病患者中显示出有益的作用。n-6 脂肪酸的作用仍有争议。本研究的目的是研究花生四烯酸(AA)、欧米茄-6 脂肪酸和十二碳五烯酸 (EPA), 欧米茄-3 FA,对单核细胞和内皮细胞之间的相互作用的影响。

 

设计

我们使用内皮细胞(ECs(EA.hy.926)和单核细胞(人类白血病粒细胞U937)的细胞模型。汇合的ECsAAEPA治疗,在肿瘤坏死因子-α(TNF-α)或只是载体中4h24 h。观察单核细胞对内皮单层的粘附。对基因表达,进行逆转录,然后进行实时定量的聚合酶链反应观察。

 

结果

在欧米茄-6脂肪酸存在时,在TNF-ALPHA存在或不存在的情况下,在4h24h时的单核细胞对内皮层的粘附明显增加。单核细胞对内皮层的粘附在欧米茄-3存在时,在24h 单核细胞对内皮层的粘附明显减少。经过欧米茄-6处理后,内皮细胞中的细胞间粘附分子1,血管细胞粘附分子1,E-Selectin,白介素6, TNF-α明显增加。

 

结论

我们的结论是,AA能增强炎症反应,增强内皮细胞(ECs)在体外结合单核细胞的能力。环境保护署导致了环境保护署能力的下降。926结合单核细胞,虽然效果看起来比较温和。综上所述,这些数据表明欧米茄-6脂肪酸可以增强炎症和动脉粥样硬化的早期事件。

 

 

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Effects of fatty acids on endothelial cells: inflammation and monocyte adhesion

脂肪酸对内皮细胞的影响:炎症和单核细胞粘附- ScienceDirect https://www.sciencedirect.com/science/article/pii/S0022480412003757

Author links open overlay panelS. MarleneGrenonMDCMabcJesusAguado-ZunigaBSabJason P.HattonPhDbChristopher D.OwensMDacMichael S.ConteMDaMillieHughes-FulfordPhDbd

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https://doi.org/10.1016/j.jss.2012.04.010Get rights and content

Abstract

Background

 

Diet is known to have an important impact on cardiovascular health. n-3 Fatty acids (FAs), found in high quantity in fish oil, have demonstrated beneficial effects in patients with coronary artery disease. The role of n-6 FAs remains more controversial. The objective of this study was to examine the effect of arachidonic acid (AA), an n-6 FA, and eicosapentanoic acid (EPA), an n-3 FA, on the interaction between monocytes and endothelial cells (ECs).

 

Design

 

We used a cellular model of ECs (EA.hy.926) and monocytes (human leukemic myelomonocytic U937). Confluent ECs were treated with AA or EPA, in the presence of tumor necrosis factor-alpha (TNF-α) or vehicle alone for either 4 or 24 h. Adhesion of monocytes to the endothelial monolayer was performed. For gene expression, reverse transcription, followed by real-time quantitative polymerase chain reaction, was performed.

 

Results

 

There was a significant increase in adhesion of monocytes to the endothelial monolayer in the presence of n-6 FAs, both in the presence and in the absence of TNF-α at 4 and 24 h. The adhesion of monocytes to the endothelial monolayer was decreased with n-3 FAs at 24 h. Intercellular adhesion molecule 1, vascular cell adhesion molecule 1, E-Selectin, Interleukin 6, and TNF-α were significantly increased in ECs treated with n-6 FAs.

 

Conclusions

 

We conclude that AA increases inflammation and enhances the ability of ECs to bind monocytes in vitro. EPA leads to a decrease in the ability of EA.hy.926 to bind monocytes, although the effect appears more modest. Taken together, these data indicate that the n-6 FA AA could potentiate inflammation and early events of atherosclerosis.

 

Effects of fatty acids on endothelial cells: inflammation and monocyte adhesion - ScienceDirect  https://www.sciencedirect.com/science/article/pii/S0022480412003757