缺镁容易导致骨质疏松 ~一氧化氮介导低镁成骨细胞样抑制细胞增殖

Nitric oxide mediates low magnesium inhibition of osteoblast-like cell proliferation

适量摄入镁(Mg)对骨细胞的活动很重要，有助于预防骨质疏松症（OSTEOPOROSIS)。

由于 镁(Mg)对成骨细胞有丝分裂作用，以及骨质疏松症检测到成骨细胞增殖减少，我们研究了不同浓度的细胞外镁（Mg）对成骨细胞样SaOS-2细胞行为的影响。

我们发现，低Mg通过诱导型一氧化氮合酶(iNOS)的上调，通过增加一氧化氮的释放来抑制SaOS-2细胞的增殖。

实际上，对iNOS抑制剂l-N6-(imino乙基)-赖氨酸- hcl的药理抑制和小干扰RNA对iNOS的遗传沉默都恢复了细胞的正常增殖速度。

由于适量诱导一氧化氮足以增强骨吸收，而成骨细胞增殖的相对缺乏会导致其活性不足，因此我们得出结论，维持镁（Mg）稳态与确保成骨细胞功能相关，从而预防骨质疏松症。

Nitric oxide mediates low magnesium inhibition of osteoblast-like cell proliferation

Author links open overlay panelMarziaLeidiaFedericaDelleraaMassimoMariottiaGiuseppeBanfibCalogeroCrapanzanocWalterAlbisettidJeanette A.M.Maiera

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https://doi.org/10.1016/j.jnutbio.2011.06.016Get rights and content

Abstract

An adequate intake of magnesium (Mg) is important for bone cell activity and contributes to the prevention of osteoporosis. Because (a) Mg is mitogenic for osteoblasts and (b) reduction of osteoblast proliferation is detected in osteoporosis, we investigated the influence of different concentrations of extracellular Mg on osteoblast-like SaOS-2 cell behavior. We found that low Mg inhibited SaOS-2 cell proliferation by increasing the release of nitric oxide through the up-regulation of inducible nitric oxide synthase (iNOS). Indeed, both pharmacological inhibition with the iNOS inhibitor l-N6-(iminoethyl)-lysine-HCl and genetic silencing of iNOS by small interfering RNA restored the normal proliferation rate of the cells.

Because a moderate induction of nitric oxide is sufficient to potentiate bone resorption and a relative deficiency in osteoblast proliferation can result in their inadequate activity, we conclude that maintaining Mg homeostasis is relevant to ensure osteoblast function and, therefore, to prevent osteoporosis.

https://www.sciencedirect.com/science/article/pii/S0955286311002208