过氧化氢气体雾化灭菌系统灭活鸭乙型肝炎病毒：实验室和“使用中”测试

　

Inactivation of duck hepatitis B virus by a hydrogen peroxide gas plasma sterilization system: Laboratory and 'in use' testing

 

　

 

医院感染杂志上的文章41（4）：317  -  22·1999年5月，46篇阅读

DOI：10.1053 / jhin.1998.0516·资料来源：PubMed

 

人类乙型肝炎病毒（HBV）是医院感染的重要原因，可通过受污染的仪器传播。然而，由于病毒不能在实验室中培养，因此难以对由HBV污染的材料和设备进行灭菌效果的测试。在这项研究中，我们旨在评估低温，过氧化氢气体 雾化灭菌器（Sterrad，Advanced Sterilization Products，Irvine California，）灭活鸭乙型肝炎病毒（DHBV）的能力。在使用干燥DHBV到玻璃过滤器载体并暴露于过氧化氢气体 雾化灭菌过程的一半的实验室功效研究中，病毒滴度降低了10（7）或更高，处理后在载体上未检测到感染性。

　

使用实验性污染DHBV的腹腔镜进行使用中的研究，以模拟连续患者之间可能的感染传播。暴露于过氧化氢气体等血浆灭菌过程后，尽管有明显的血液污染（N = 8），但未发生腹腔镜DHBV感染的传播，而未 处理的腹腔镜（阳性对照）的传输率为100％（26/26）水洗后的仪器使用率为63％（7/11）。总之，过氧化氢 气体雾化灭菌过程完全灭活DHBV，这是Hepadna病毒组的代表。

　

　

Article in Journal of Hospital Infection 41(4):317-22 · May 1999 with 46 Reads

DOI: 10.1053/jhin.1998.0516 · Source: PubMed

 

Human hepatitis B virus (HBV) is an important cause of nosocomial infections and can be transmitted by contaminated instruments. However, tests of the efficacy of sterilization of materials and equipment contaminated by HBV are difficult to perform because the virus cannot be cultured in the laboratory. In this study, we aimed to evaluate the capability of a low temperature, hydrogen peroxide gas plasma sterilizer (Sterrad, Advanced Sterilization Products, Irvine California,) to inactivate duck hepatitis B virus (DHBV). In laboratory efficacy studies using DHBV dried on to glass filter carriers and exposed to one-half of the hydrogen peroxide gas plasma sterilization process, there was a 10(7) or greater decrease in the viral titer, with no infectivity detected on the carriers after treatment. In-use studies were performed using a laparoscope that was experimentally contaminated with DHBV to mimic the possible transmission of infection between successive patients. Following exposure to the hydrogen peroxide gas plasma sterilization process no transmission of DHBV infection from the laparoscope occurred despite obvious visual soiling with blood (N = 8) while the transmission rate for the unprocessed laparoscope (positive control) was 100% (26/26), and that for instruments after a water wash was 63% (7/11). In conclusion the hydrogen gas plasma sterilization process completely inactivates DHBV a representative of the hepadna group of viruses.

 

https://www.researchgate.net/publication/12907567_Inactivation_of_duck_hepatitis_

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