抗微生物的光动力疗法：以叶绿素为光敏剂杀死变形链球菌
Antimicrobial Photodynamic Therapy: Chlorophyll as a Photosensitizer Against S. mutans.
目的：本研究的目的是通过使用液体叶绿素作为光敏剂和发光二极管（LED）来研究抗生素光动力疗法（aPDT）对变形链球菌的生存能力。
方法：实验组有三个：G1：不接触LED，也不接触液体叶绿素（n = 7）; G2：仅暴露于液体叶绿素而不暴露于LED（n = 7）; G3：暴露于LED和液体叶绿素（n = 7）。将所有组的细菌生长转移到培养皿中，并将它们在微需氧条件下培养48小时。然后计数通过细菌接种物的稀释液102和103获得的菌落形成单位（CFU）。结果已提交ANOVA和Bonferroni检验（p <0.05）。
结果：在叶绿素与LED的关联中，CFU的降低有统计学意义。仅在叶绿素存在下CFU评分也降低。
结论：使用LED的液体叶绿素和光动力疗法的关联能够减少变形链球菌的数量。这一结果对于在即将到来的未来为临床方案提供龋齿预防的新视角非常重要。
Antimicrobial Photodynamic Therapy: Chlorophyll as a Photosensitizer Against S. mutans.
Conference Paper · June 2012 with 21 Reads
Conference: IADR General Session 2012
Objective: The aim of this work was to investigate the antimicrobial photodynamic therapy (aPDT) by using liquid chlorophyll as photosensitizer and Light Emitting Diode (LED) in the viability of S. mutans. Method: There were three experimental groups: G1: no exposure to LED nor to liquid chlorophyll (n=7); G2: with exposure only to liquid chlorophyll and not to LED (n=7); G3: with exposure to both LED and liquid chlorophyll (n=7). The bacterial growth of all groups was transferred to Petri plaques, and these were incubated in microaerophilic conditions for 48 hours. The colony-forming units (CFU), obtained by dilutions 102 and 103 of the bacterial inoculum, had been then counted. The results had been submitted to ANOVA and Bonferroni tests (p<0.05). Result: There was statistically significant reduction of CFU in the association of chlorophyll to LED. The CFU score was also reduced in the presence of chlorophyll only. Conclusion: The association of liquid chlorophyll and Photodynamic Therapy using LED was able to reduce the number of S. mutans. This result is important in order to bring a new perspective for clinical protocol to caries prevention in a forthcoming future.