Low Red Blood Cell Vitamin C Concentrations Induce Red Blood Cell Fragility: A Link to Diabetes Via Glucose, Glucose Transporters, and Dehydroascorbic Acid
Molecular and Clinical Nutrition Section, Digestive Diseases Branch, Intramural Research Program, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health (NIDDK, NIH)
September 2015, Available online 3 October 2015.
Abstract
Strategies to prevent diabetic microvascular angiopathy focus on the vascular endothelium. Because red blood cells (RBCs) are less deformable in diabetes, we explored an original concept linking decreased RBC deformability to RBC ascorbate and hyperglycemia. We characterized ascorbate concentrations from human and mouse RBCs and plasma, and showed an inverse relationship between RBC ascorbate concentrations and deformability, measured by osmotic fragility. RBCs from ascorbate deficient mice were osmotically sensitive, appeared as spherocytes, and had decreased β-spectrin. These aberrancies reversed with ascorbate repletion in vivo. Under physiologic conditions, only ascorbate's oxidation product dehydroascorbic acid (DHA), a substrate for facilitated glucose transporters, was transported into mouse and human RBCs, with immediate intracellular reduction to ascorbate. In vitro, glucose inhibited entry of physiologic concentrations of dehydroascorbic acid into mouse and human RBCs. In vivo, plasma glucose concentrations in normal and diabetic mice and humans were inversely related to respective RBC ascorbate concentrations, as was osmotic fragility. Human RBC β-spectrin declined as diabetes worsened. Taken together, hyperglycemia in diabetes produced lower RBC ascorbate with increased RBC rigidity, a candidate to drive microvascular angiopathy. Because glucose transporter expression, DHA transport, and its inhibition by glucose differed for mouse versus human RBCs, human experimentation is indicated.
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Abbreviations
AAascorbic acidDHAdehydroascorbic acidGLUTfacilitated glucose transporterGulo-/-gulonolactone oxidase knockout mouse unable to synthesize ascorbatePBSphosphate buffered salineRBCsred blood cellsRIPAWestern blot cell lysis bufferSVCTsodium-dependent vitamin C transporterTCEPTris(2-carboxyethyl)phosphine3-O-MG3-O-methylglucoseWTwildtype mouse
Keywords
Ascorbic AcidDehydroascorbic AcidRed Blood CellsDiabetesGlucose Transportβ-Spectrin血红细胞维生素C浓度过低导致血红细胞脆弱:通过葡萄糖、葡萄糖转运体和脱氢抗坏血酸与糖尿病有关
美国国家卫生研究院糖尿病、消化和肾脏疾病研究所内部研究项目消化疾病分会分子与临床营养科
预防糖尿病微血管病变的策略主要集中在血管内皮。由于糖尿病患者的红细胞变形能力较弱，我们探索了红细胞变形能力下降与红细胞抗坏血酸和高血糖之间的联系。我们对人、小鼠红细胞和血浆中的抗坏血酸浓度进行了分析，发现抗坏血酸浓度与变形能力(通过渗透脆性来衡量)呈反向关系。抗坏血酸缺乏小鼠的红细胞具有渗透敏感性，表现为球形细胞，并具有较低的beta-spectrin。体内抗坏血酸充盈可逆转这些异常。在生理条件下，只有抗坏血酸的氧化产物脱氢抗坏血酸(DHA)被运输到小鼠和人类红细胞中，细胞内立即还原为抗坏血酸。在体外，葡萄糖抑制生理浓度的脱氢抗坏血酸进入小鼠和人红细胞。在体内，正常和糖尿病小鼠和人的血糖浓度与各自的红细胞抗坏血酸浓度呈负相关，渗透脆性也是如此。随着糖尿病的恶化，人红细胞的beta-spectrin下降。综上所提，糖尿病患者高血糖可产生较低的红细胞抗坏血酸，并增加红细胞硬度，这是微血管病变的候选病因。由于葡萄糖转运蛋白的表达、DHA的转运以及葡萄糖对其的抑制作用在小鼠和人红细胞中是不同的，因此我们建议进行人体实验。
假设来源和相关的维生素C在小鼠和人类的浓度
关于抗坏血酸、糖尿病和红细胞生成素的最初线索来自于对无法合成维生素c的小鼠(Gulo−/−)的全血的意外观察(Gulo−/−)。当全血被离心，来自未补充维生素C的小鼠的样本有明显的溶血(图1A)。为了防止溶血，慢速离心时间更长，可以测定血浆中抗坏血酸的浓度。补充维生素C的Gulo−/−小鼠抗坏血酸值为~ 74°M，未补充维生素C的Gulo−/−小鼠为~ 4°M。溶血仅发生在血浆低抗坏血酸浓度的样本中，表明红细胞内维生素C含量低是一个原因。为了探索这种可能性，我们直接测定了小鼠红细胞维生素C浓度，并与同一全血标本的血浆浓度进行比较，发现二者呈线性关系(R = 0.91, p < 0.01)(图1B)。渗透脆性测量表明，与对照组相比，抗坏血酸耗竭小鼠的脆性增加，而补充抗坏血酸则相反(图2F)。与对照组相比，耗竭再补充的Gulo−/−小鼠的RBC外观无明显差异(补充图1C)。
3.3。与糖尿病的潜在关系:脱氢抗坏血酸和抗坏血酸转运到人类和小鼠红细胞
抗坏血酸盐之间的潜在联系,红血球,糖尿病可能物种运送到红细胞表面氧化抗坏血酸盐,或脱氢抗坏血酸(DHA),然后进入通过葡萄糖转运蛋白和立即被细胞还原(曼和牛顿休斯和Maton, 1968年,1975年,比安奇和玫瑰,1986年,Mendiratta et al ., 1998)。为了描述人类和小鼠红细胞中的转运物种，我们使用估计的DHA生理血浆浓度，即1-5%的抗坏血酸盐(Dhariwal等人，1991,Lykkesfeldt, 2000);生理血浆抗坏血酸浓度(Levine et al.， 1996, Levine et al.， 2001);新合成DHA (Rumsey等，1997,Corpe等，2013);以及最近开发的红细胞抗坏血酸测定方法(Li et al.， 2012)。细胞外DHA作为底物测定来验证它的存在(见图3和补充图。2)。在人类红血球,DHA在2μM热切地运输,这样在2分钟内几乎所有的DHA摩尔在媒体易位到红细胞表面,减少抗坏血酸盐(图3,补充图3传奇)。在相同的条件下，未转运抗坏血酸盐(图3A)。使用DHA和抗坏血酸浓度(补充图3A)和人类红细胞，得到了类似的结果:只有DHA被运输。小鼠红细胞也只转运DHA而不转运抗坏血酸盐，但转运率比人类红细胞低10-20倍(图3B;补充图3 b)。仅在红细胞中发现了抗坏血酸(Li et al.， 2012) (supplementary Fig. 4A,B)。3.4。葡萄糖在体外抑制DHA进入红细胞，转运蛋白表达，以及葡萄糖在体内对红细胞抗坏血酸浓度和渗透脆性的影响
由于DHA在体外小鼠红细胞中的转运和抑制作用较人红细胞弱，葡萄糖是否会影响小鼠红细胞中抗坏血酸的浓度尚不确定。我们使用AZIP脂肪萎缩型糖尿病小鼠模型(Moitra et al.， 1998)进行研究。这些老鼠有稳定的高血糖，但在夜间禁食后又出现逆转。在AZIP和WT对照小鼠中，未禁食和禁食小鼠的红细胞抗坏血酸和血糖呈负相关(图5A)。高血糖诱导的RBC抗坏血酸降低发生在100 - 200 mg/dl (5.5-11.1 mM)之间，这是糖尿病的关键范围(美国糖尿病协会:Position Statement, 2014，美国糖尿病协会:Position Statement, 2015)。由于小鼠和人类红细胞上这些独特的过剩转运蛋白差异，我们对健康和糖尿病受试者的红细胞进行了抗坏血酸、渗透脆性和光谱蛋白的鉴定。这些受试者红细胞中抗坏血酸与空腹血糖呈负相关(图7A，补充图7);以及糖尿病的渗透脆性和严重程度(图7B)。在渗透脆性方面，健康受试者与控制不良的糖尿病受试者(血红蛋白A1C > 7.8)相比，p < 0.05。临床数据描述红细胞渗透脆性与抗坏血酸盐浓度与老鼠的发现是一致的(图2)。同样,临床数据表明高血糖是降低红细胞抗坏血酸盐浓度与老鼠的发现是一致的(图5)。与健康受试者相比,β与温和的受试者的血影蛋白逐渐下降和糖尿病控制不佳,而α血影蛋白不变(图7 c, D,E).图7E显示了这些受试者的红细胞抗坏血酸值之间存在一种线性关系,血浆和红细胞抗坏血酸盐与高血糖以及正常(补充图7)。数据显示在这种方式中,又一次重复,红细胞抗坏血酸盐与高血糖与正常相比显著降低(补充图7)。在将来的临床试验中，高血糖如何剧烈地营养红细胞抗坏血酸最好在同一个病人，在血糖正常和高血糖状态时测定。
讨论
在小鼠和人类中，数据表明红细胞中抗坏血酸浓度与高血糖呈负相关。体内DHA和葡萄糖之间的竞争是一个有吸引力的解释，但有多种途径可以导致糖尿病患者的红细胞抗坏血酸水平降低。对于红细胞，糖尿病可导致:GLUT1转运蛋白表达下调;过度的转运体类型的变化;DHA 1 过度的转运蛋白的活性降低，即氧化损伤(Nishikawa et al.， 2000, Yu et al.， 2006, Wang et al.， 2012);DHA与葡萄糖浓度升高之间的竞争;加速抗坏血酸的利用，偶联红细胞中山梨醇的形成或高血糖引起的外源性氧化剂的生成(Nishikawa等，2000,Yu等，2006,Wang等，2012);或者改变维生素C -维生素E的循环。除红细胞外，糖尿病患者血浆抗坏血酸水平可能较低，原因如下:抗坏血酸摄入量较少;降低抗坏血酸的肠道吸收;肾小球滤过率(GFR)增高或SVCT1受损导致再吸收减少;或增加抗坏血酸的利用率。如果由于上述任何一种原因，血浆维生素C浓度较低，那么用于RBC的脱氢抗坏血酸就会减少。
据报道，糖尿病患者血浆中维生素C浓度较低(Will and Byers, 1996, Chen et al.， 2006)，这与盛宴后的饥荒假说一致(图8)。然而，先前的维生素C和糖尿病之间的联系并没有考虑到结构上的血管并发症，除了广泛的氧化损伤。除了这里提供的数据集，没有人认识到糖尿病人的红细胞中维生素C的浓度低于预期。不幸的是，大多数已有的糖尿病患者血浆维生素C浓度数据被试验伪影所混淆;通过之前无法测量红细胞抗坏血酸;无法在血浆或红细胞中直接测定脱氢抗坏血酸(Will和Byers, 1996, Li等人，2012,Dhariwal等人，1991,Lykkesfeldt, 2000)。令人惊讶的是，现代血液学教科书甚至没有描述红细胞中存在维生素C (Kaushansky et al.， 2010, Greer et al.， 2013)。据我们所知，这里显示的红细胞与血浆维生素C浓度的数据是迄今为止在健康和糖尿病患者中最全面的。
How hyperglycemia dynamically affects RBC ascorbate will be best determined in the same diabetic patients with and without hyperglycemia in future clinical experiments.
1. Introduction
Diabetes type 2 is an epidemic public health problem. Poorly controlled diabetes results in accelerated microvascular disease and chronic debilitating morbidities and mortality (Beckman et al., 2002). Diabetic microvascular angiopathy is the leading cause of blindness, end stage renal disease and amputations worldwide, as well as myocardial infarction, stroke and peripheral arterial disease.
Preventing or delaying microvascular disease could improve the lives of millions, prevent catastrophic illness, and save billions of dollars. The pathogenesis of microangiopathy in diabetes is unknown. Clinical efforts are based on glycemic control. The research focus of some prevention efforts is the endothelium and its role in protecting blood vessels (Fioretto et al., 2010, Wong et al., 2010). Vascular smooth muscle abnormalities, platelet dysfunction, abnormal coagulation and impaired vascular repair are other pathologies proposed to lead to diabetic vasculopathy (Beckman et al., 2002, Cubbon et al., 2013). Oxidants generated by hyperglycemia within endothelial cells (Nishikawa et al., 2000) or other vascular cells may initiate endothelial cell damage (Yu et al., 2006, Wang et al., 2012).
Endothelial cell damage secondary to hyperglycemia can be one initiator of diabetic microangiopathy by impairing oxygen delivery, resulting in microvascular hypoxia. Another plausible pathway is that oxidants generated by hyperglycemia, from endothelial cells or others, impair oxygen delivery by affecting the delivery system itself: red blood cells (RBCs). In fact, substantial evidence indicates that diabetes induces changes in RBC structure and function through a progressive decline in RBC deformability (Peterson et al., 1977, McMillan et al., 1978, Kamada et al., 1992, Virtue et al., 2004, Diamantopoulos et al., 2004, Brown et al., 2005, Shin et al., 2007, Kung et al., 2009, Keymel et al., 2011, Buys et al., 2013). RBC deformability is vital to RBC function, and plays a major role in microvascular flow. Impaired deformability adversely affects capillary perfusion (Simchon et al., 1987, Parthasarathi and Lipowsky, 1999). Consistent with decreased deformability, diabetes is associated with increased RBC fragility and decreased RBC survival (Peterson et al., 1977, Parthasarathi and Lipowsky, 1999, Virtue et al., 2004, Kung et al., 2009). Because stiffer RBCs may compromise the microcirculation and oxygen delivery, strategies to improve RBC deformability could modify microvascular hypoxia, with direct clinical implications.
In this regard, there are underappreciated links between RBCs, vitamin C, and diabetes. Multiple reports describe lower vitamin C concentrations in diabetic subjects, especially those with microvascular complications such as retinopathy and nephropathy (Som et al., 1981, Ali and Chakraborty, 1989, Sinclair et al., 1991, Will and Byers, 1996, Lindsay et al., 1998, Cunningham, 1998, Chen et al., 2006). However, many datasets utilized unreliable vitamin C assays, making it difficult to interpret findings (Will and Byers, 1996, Padayatty et al., 2003). Diabetic vascular disease and vitamin C deficiency were tied together in an early hypothesis (Mann and Newton, 1975), but it lacked mechanism and supportive evidence. Consistent with a hypothesized role for a RBC deformability defect in diabetes, anemia and hemolysis are manifestations of vitamin C deficiency in humans, and in mice (Gulo−/−) unable to synthesize the vitamin (Chazan and Mistilis, 1963, Hart et al., 1964, Cox, 1968, Maeda et al., 2000). Unfortunately, deformability measures were not described in vitamin C deficient patients, and their clinical data are confounded by co-existent vitamin deficiencies.
Here we couple original links between diabetes and vitamin C with RBCs as a key cell type; oxidized vitamin C (dehydroascorbic acid, DHA) as a key transported substrate; and the chemical structure similarity between DHA and glucose (Vera et al., 1993, Rumsey et al., 1997, Corpe et al., 2013). For nearly all tissues, ascorbate transport is mediated by sodium-dependent vitamin C transporter SVCT2 (Sotiriou et al., 2002). However, SVCT2 is absent from RBCs (May et al., 2007). Because RBCs contain ascorbate (Li et al., 2012), another transport mechanism exists. It is likely that the product of ascorbate oxidation, dehydroascorbic acid, is transported on facilitated glucose transporters (GLUTs) and immediately reduced to ascorbate within RBCs (Hughes and Maton, 1968, Bianchi and Rose, 1986, Mendiratta et al., 1998). Based on expressed transporter data, hyperglycemia from diabetes could inhibit dehydroascorbic acid entry into RBCs (Vera et al., 1993, Rumsey et al., 1997). Some data do not support this rationale, but experiments were performed using DHA concentrations 2–3 orders of magnitude above physiological concentrations, and indirect assays that did not account for substrate degradation (Montel-Hagen et al., 2008a, Sage and Carruthers, 2014). Lower DHA concentrations could not be investigated due to assay limitations, also precluding accurate RBC ascorbate measurements (May et al., 2001, Montel-Hagen et al., 2008a, Li et al., 2012).
Utilizing a recently developed ultrasensitive assay for vitamin C in RBCs and physiologic transport conditions (Li et al., 2012), we propose an original multicomponent hypothesis linking vitamin C to diabetes. The parts of the hypothesis investigated here are whether low ascorbate concentrations occur in RBCs in comparison with other cells; whether low ascorbate concentrations in RBCs have a consequence; and whether and how low ascorbate RBC concentrations can be coupled to hyperglycemia in vitro and in vivo.
2. Methods
2.1. Materials
Ascorbic acid was purchased from Sigma/Aldrich. Dehydroascorbic acid was synthesized de novo from ascorbate immediately before each experiment (Li et al., 2012, Corpe et al., 2013). Antibodies were obtained from Abcam (Cambridge MA), Santa Cruz Biotechnology (Dallas TX), and Novus Biologicals (Littleton, CO). Antibodies from Abcam: anti-GLUT 1 antibody (ab652), anti-GLUT 2 antibody(ab54460), anti-GLUT 3 antibody (ab41525), anti-GLUT 4 antibody (ab654), anti- β actin antibody (ab6276), anti- β 1 spectrin (ab2808), and anti-(α + β) spectrin (ab11182). Antibodies from Santa Cruz Biotechnology: anti-Ankyrin-1 (sc-12,733). Antibodies from Novus Biologicals: anti-protein 4.2 (NBP1-56,647). All other chemicals were highest purity grade available commercially.
2.2. Mice and Blood Samples from Mice
Animal experiments were approved by the Animal Care and Use Committee NIDDK, NIH, and were conducted in accordance with NIH guidelines. Unless otherwise indicated, mice were 10–14 week old males with free access to food and water, and were maintained on regular chow diet (NIH-07) without detectable ascorbate (detection limit 10 nM). Mice were type C57BL/6 (wildtype, WT) (Charles River Laboratories, Wilmington, MA, USA); transgenic AZIP mice (original FVB/N A-ZIP/F-1 line) Moitra et al., 1998); and gulonolactone oxidase (Gulo+/−) mice (Mutant Mouse Regional Resource Center, University of California at Davis, USA), bred as described (Maeda et al., 2000). Plasma and RBCs were obtained from mouse whole blood as described (Li et al., 2012), with the modification that samples were centrifuged at 200 x g for 5 min due to hemolysis (see Fig. 1 and results). When ascorbate was provided, mice received it via drinking water, which was changed daily, or via gavage where indicated. See supplementary methods for additional details.
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Fig. 1. Hypothesis origin and vitamin C concentrations in RBCs and plasma.
A. Hypothesis origins: findings from centrifugation of mouse whole blood. For ascorbate values, samples were obtained from 5 Gulo−/− mice supplemented with ascorbate 1 g/L in drinking water for 12 weeks and 5 Gulo−/− mice not supplemented with ascorbate for 12 weeks, plasma concentrations indicated in the panel. Gulo−/− mice do not make ascorbate. When ascorbate was provided, mice received it via drinking water, which was changed daily.
B. Mouse RBC ascorbate as a function of plasma ascorbate. 12 wildtype (WT) mice were unsupplemented for 14 weeks (); 10 Gulo−/− mice were unsupplemented from 0 to 14 weeks (); and 5 Gulo−/− mice were supplemented with 1 g/L ascorbate in drinking water from 0 to 17 weeks (●). Each symbol represents a separate blood sample. R = 0.91, p < 0.01.
C. Human RBC ascorbate as a function of plasma ascorbate in 153 healthy blood donors, ascorbate measured by HPLC with coulometric electrochemical detection (Li et al., 2012). Each sample was measured in triplicate, samples without error bars indicate SD was less than symbol size. R = 0.82, p < 0.02.
D. Human mononuclear cell ascorbate (all non-open circle symbols) as a function of plasma ascorbate, ascorbate measured by HPLC. In-patient healthy subjects (6 men and 13 women) who were at steady state for vitamin C doses of 30, 60, 100, 200, 400, 1000, and 2500 mg in two divided doses daily underwent apheresis with elutriation of cell-enriched product to obtain mixed mononuclear cells, as described (Levine et al., 1996, Levine et al., 2001). Each symbol represents a different subject. For each subject, samples were obtained at 1–5 different vitamin C doses at steady state for that dose (Levine et al., 1996, Levine et al., 2001).
2.3. Human Subjects and Samples
Clinical research was approved by the Institutional Review Board, NIDDK/NIAMS, NIH, and conducted in accordance with NIH guidelines. Blood and cell samples from healthy subjects (NIH Protocols 04-DK-0021; 99-CC-0168; and 92-DK-0033) and diabetic subjects (NIH Protocol 04-DK-0021) were obtained and processed as described (Levine et al., 1996) (Li et al., 2012).
2.4. Erythrocyte Osmotic Fragility
RBC deformability is related to RBC osmotic fragility (Clark et al., 1983). RBC osmotic fragility based on resistance of RBCs to lysis as a function of decreasing NaCl concentration was performed as described (Parpart et al., 1947) with modifications (see supplementary methods).
2.5. Dehydroascorbic Acid and Ascorbate Transport
2.5.1. Preparation of RBCs
RBCs were prepared as described (Li et al., 2012), with centrifugation modifications above.
2.5.2. Transport of Dehydroascorbic Acid and Ascorbate into Mouse and Human RBCs
Human (50 μL) or mouse (30 μL) RBCs were added to PBS (450 μL for human RBCs, 270 μL for mouse RBCs) containing 5 mM glucose and freshly prepared ascorbate, [14C]ascorbate, dehydroascorbic acid, or [14C]dehydroascorbic acid. RBCs and supernatants were prepared and analyzed as described previously (Li et al., 2012).
2.6. Inhibition of 3-O-[3H]MG and [14C]DHA Uptake into Mouse and Human RBCs
2.6.1. Inhibition of 3-O-[3H]MG and [14C]DHA Uptake into Mouse and Human RBCs by Unlabeled 3-O-MG
Human RBCs 2 mL prepared as above were incubated with 20 mM 3-O-MG in PBS (final volume 20 mL) for 20 min at 37 °C (See supplementary methods). After centrifugation at 500 x g for 5 min, the supernatant was removed and preloaded RBCs placed on ice until use. For competition between unlabeled and 3-O-[3H]MG, RBC 50 μL were added to 450 μL PBS on ice containing 1 μCi/mL 3-O-[3H]MG and one of the following concentrations of unlabeled 3-O-MG: 0.005, 1, 2.5, 5, 10, 20, 30 mM. For competition between unlabeled 3-O-MG, and [14C]DHA, RBC 50 μL were added to 450 μl PBS on ice containing freshly prepared 5 μM [14C]DHA and one of the following concentrations of unlabeled 3-O-MG: 0.005, 1, 2.5, 5, 10, 20, 30 mM. After incubation on ice for 1 min, 1 mL ice-cold stop buffer (10 μM cytochalasin B in PBS) was added, and the mixture centrifuged at 200 x g for 3 min at 4 °C. The supernatant was discarded, and RBCs were washed twice more at 4 °C. From the resulting RBC pellet, 40 μL were added to 360 μL ultrapurified water and the mixture repetitively pipetted for at least 10 s. The resulting lysate was transferred to a centrifugal filtration device (Amicon Ultra 0.5 ml 10 K Ultracell, Millipore) (Li et al., 2012), and centrifuged at 14,000 x g for 15 min at 4 °C. Filtrate 100 μL was added to 5 mL scintillation fluid followed by scintillation spectrometry analyses.
Mouse RBC experiments were conducted similarly, with the following modifications. For pre-loading RBCs with 3-O-MG, 0.4 mL washed RBCs were re-suspended in 3.6 mL PBS containing 20 mM 3-O-MG at 37 °C for 20 min, with removal of supernatant and placement on ice until use. For 3-O-MG competition experiments with 3-O-[3H]MG, 40 μL pre-loaded RBCs were added to 360 μL PBS at 37 °C containing 1 μCi/mL 3-O-[3H]MG with 5 μM or 30 mM unlabeled 3-O-MG. For 3-O-MG competition experiments with [14C] DHA, 40 μL pre-loaded RBCs were added to 360 μL PBS at 37 °C containing freshly prepared 5 μM [14C] DHA with 5 μM or 30 mM unlabeled 3-O-MG. After RBC addition, mixtures were incubated at 37 °C for 1 min, followed by addition of ice-cold stop buffer (PBS with 10 μM cytochalasin B), washing, lysis, filtration, and analyses by scintillation spectrometry.
2.6.2. Inhibition of 3-O-[3H]MG and [14C]DHA Uptake into Human and Mouse RBCs by Cytochalasin B
After washed human RBCs 50 μL were re-suspended in 450 μL PBS with 20 mM 3-O-MG for 20 min at 37 °C as above, 0.5 μL of either dimethyl formamide alone or containing cytochalasin B was added. Final cytochalasin B concentration was 20 μM. After RBCs were incubated for an additional 15 min at 37 °C, they were pelleted by centrifugation at 50 x g for 5 min at 4 °C and 400 μL of supernatant was removed. The loose RBC pellet was re-suspended with 500 μL of pre-chilled PBS containing either 3-O-[3H]MG (5 μM, 1 μCi/mL) or [14C]dehydroascorbic acid (DHA)(freshly prepared, 5 μM, 0.027 μCi/mL), and incubated for 15, 30, 45 or 60 s on ice. At the end of the incubation time, 1 mL of prechilled PBS containing 20 mM 3-O-MG and 20 μM cytochalasin B was added, and RBCs were washed 3 times at 4 °C using this solution. As above, the final pellet was lysed in water, filtered using a centrifugal filtration device, and analyzed by scintillation spectrometry.
For mouse RBCs, experiments were conducted as for human RBCs with the following modifications: time point was one minute, temperature was 37 °C.
2.7. Xenopus Laevis Oocyte Transport Assay
cRNAs (complementary RNAs) of human GLUT 1 or mouse Glut 3 were prepared by in vitro transcription (mMessage mMachine, Ambion). Xenopus laevis oocytes were isolated and injected with cRNAs as described (Corpe et al., 2013), see supplementary methods for details.
2.8. Western Blot
For sample preparation, one volume of mouse or human intact red cells were washed three times with cold PBS before lysis using 4 volumes of modified RIPA buffer (50 mM Tris, pH 7.4, 150 mM NaCl, 0.1% SDS, 0.25% Na deoxycholate, 2% Triton-X100, 1 mM PMSF, 2uM leupeptin). Western blot was performed on RBC lysates according to manufacturer's instructions (Life Technologies, Carlsbad CA), see supplementary methods for details.
Anti-GLUTs (1–4) antibodies were all polyclonal antibodies. Based on supplier information, antibodies detected transporters from mice, rats, and humans. For GLUT Western blots of RBC lysates from human and mice: if no or weak bands were detected in either species, a positive control of the species (human/mouse cell lines or isolated mouse tissue) was added to determine whether the antibody recognized epitopes across species, see supplementary methods for details. GLUT 1 Western blots were developed using normal sensitivity substrate (Pierce ECL Western blot Substrate, Thermo), while other GLUT Western blots were developed using higher sensitivity substrate (SuperSignal West Pico Chemiluminescent Substrate, Thermo). RBC ghosts were prepared as described (Steck and Kant, 1974). For mouse RBCs, anti- β 1 spectrin antibody was used to detect β-spectrin, and anti-(α + β) spectrin antibody was used to detect α-spectrin. For human RBCs, anti-(α + β) spectrin antibody was used to detect β-spectrin and α-spectrin.
2.9. Confocal Microscopy
RBCs were fixed using 0.1% glutaraldehyde, stained with Alexa Fluor 488 phalloidin (5 units/mL), and analyzed by confocal microscopy (Yau et al., 2012). Confocal laser scanning microscopy analysis was performed with a Duo-Scan system (LSM 5 LIVE; Carl Zeiss Inc., Thornwood, NY) using a 63 × 1.4 NA oil immersion objective. Laser 489 nm line was applied for excitation and emission was collected with 518 nm filter. Images were acquired using ZEN 2007 software (Carl Zeiss, Inc.). Whole cell and biconcave area diameters were determined by drawing a horizontal line through the center of each target RBC and calculating the distances between two points where fluorescent intensities were most different.
2.10. Ascorbic Acid, Dehydroascorbic Acid, and Glucose Analyses
Whole blood glucose was measured by established methods, see supplementary methods for details. Briefly, mouse whole blood glucose measurements were based on conversion of glucose to gluconolactone (Accu Chek, Roche Diagnostics), and humans samples were measured by the hexokinase method (Sacks, 2008). Values between methods used for mouse and human samples are within 10% (Freckmann et al., 2012). Ascorbic acid and dehydroascorbic acid were measured by HPLC (high performance liquid chromatography) as described (Li et al., 2012). RBC intracellular water space was 70% of packed RBC volume (Kageyama et al., 1989, Mendiratta et al., 1998). Although RBC volume differs from mice to humans, mice have similar intracellular water space (Tanabe et al., 1986). White blood cell volumes, determined as previously described, are 0.25 μL/106 mixed mononuclear cells (Bergsten et al., 1990).
2.11. Calculations
Data displayed are mean ± S.D. unless otherwise indicated. Numbers of patients or mice are indicated in legends. Otherwise, each data point represents N of at least 3 replicates unless noted differently in figure legends. Points lacking error bars indicate S.D. was less than symbol size. Comparisons between 3 or more groups utilized 1-way ANOVA followed by Tukey's multiple comparison test (Graphpad Prism version 5.01; SigmaPlot version 13). Two groups were compared using, 2-tailed Student's t test (Excel 2010). p ≤ 0.05 or less was considered significant.
Significance versus respective control was indicated as follows: *: p ≤ 0.05; **: p ≤ 0.01; ***: p ≤ 0.005; ****: p < 0.001.
3. Results
3.1. Hypothesis Origin and Relevant Vitamin C Concentrations in Mice and Humans
The original clue linking ascorbate, diabetes and RBCs came from unexpected observations of whole blood obtained from mice (Gulo−/−) unable to synthesize vitamin C. When whole blood was centrifuged, samples from unsupplemented mice had visible hemolysis (Fig. 1A). With slower centrifugation for longer times to prevent hemolysis, plasma ascorbate concentrations could be determined. Ascorbate values for supplemented Gulo−/− mice were ~ 74 μM, and for unsupplemented Gulo−/− mice were ~ 4 μM. Hemolysis only in samples with low plasma ascorbate concentrations implicated low vitamin C within RBCs as a cause. To explore this possibility, we directly measured mouse RBC vitamin C concentrations, and when compared to plasma concentrations from the same whole blood samples, a linear relationship was found (R = 0.91, p < 0.01) (Fig. 1B).
To learn whether mouse vitamin C data were relevant to humans, we measured human plasma and RBC ascorbate concentrations (Fig. 1C). In whole blood from > 150 random healthy humans, RBC ascorbate concentration was at or below the associated plasma concentration, with a linear relationship between both compartments (R = 0.82, p < 0.02). In > 10% of subjects, RBC ascorbate values were < 20 μM, in the early vitamin C deficiency range (Levine et al., 1996, Levine et al., 2001). The concentrations in RBCs were then compared to another human circulating cell type, mononuclear cells (Fig. 1D). In contrast to RBCs (Fig. 1C), mononuclear cells had ascorbate concentrations that were > 30-fold higher than concurrent plasma concentrations (Fig. 1D). Mononuclear cell data are consistent with other cell-types (Levine et al., 1996, Levine et al., 2001).
3.2. Osmotic Fragility in Mouse RBCs in Relation to Ascorbate Concentrations and Underlying Mechanism
Based on Fig. 1, low RBC vitamin C concentrations were clinically plausible. Next steps were to investigate RBC fragility in relation to plasma and RBC vitamin C concentrations. Because low ascorbate levels are not easily obtainable in humans (Levine et al., 1996, Levine et al., 2001), we studied Gulo−/− mice (Maeda et al., 2000). We predicted that plasma and RBC ascorbate concentrations in Gulo−/− mice would be a function of ascorbate depletion or supplementation, and it would be possible to have animals with low or high values. Wildtype (WT) mice, which synthesize ascorbate, were used as controls. RBC osmotic fragility was a surrogate for RBC deformability (Clark et al., 1983). RBCs were obtained from ascorbate unsupplemented Gulo−/− mice with low plasma ascorbate concentrations (4 μM). These RBCs were more sensitive to osmotic lysis compared to supplemented Gulo−/− mice with normal plasma ascorbate concentrations (64 μM) (p < 0.05 for osmotic lysis) and compared to WT mice, with plasma ascorbate concentrations of 77 μM (p < 0.001 for osmotic lysis) (Fig. 2A). To further characterize osmotic fragility, it was necessary to use RBCs whose ascorbate concentrations varied over a wide range. This was achieved by using Gulo−/− mice and withholding ascorbate for as long as 14 weeks (Fig. 2B, see Fig. 2 legend for details). The longer Gulo−/− mice remained unsupplemented, the more plasma and RBC ascorbate values declined (supplementary Fig. 1A). Osmotic fragility was then tested in relation to these declining RBC ascorbate values (Fig. 2C). Once RBC values were < 10 μM, increased osmotic fragility occurred. These data, as in Fig. 2A, indicate that RBC fragility is inversely related to RBC ascorbate concentration.
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Fig. 2. Osmotic fragility in mouse RBCs as a function of their ascorbate concentrations and underlying mechanism.
A. Osmotic RBC fragility in mouse RBCs as a function of NaCl concentration. RBC samples were obtained from 5 WT unsupplmented mice (); 5 Gulo−/− mice without ascorbate for 12 weeks (); and 5 Gulo−/− mice supplemented with 1 g/L ascorbate for 12 weeks (●); N = 5 mice per point. The 50% lysis point for each condition (horizontal line) is determined by the vertical line of the same color. Plasma ascorbate concentrations: WT mice, 77.6 μM; Gulo−/− mice, 4.0 μM; Gulo−/− mice supplemented with 1 g/L ascorbate for 12 weeks 64.8 μM. Statistics (two-tailed T-test) for 50% lysis: WT vs. unsupplemented Gulo−/− mice, p < 0.001; unsupplemented vs supplemented Gulo−/− mice, p < 0.05. Wildtype and supplemented Gulo−/− mice were not statistically different.
B. RBC ascorbate as a function of plasma ascorbate in Gulo−/− mice also tested for RBC osmotic fragility (see Fig. 2C). Five Gulo−/− mice were supplemented with ascorbate 1 g/L for 17 weeks. Ten Gulo−/− mice were deprived of ascorbate for 0–14 weeks, followed by gavage supplementation with 0.2 mg of ascorbate in 100 μL water once at 14 weeks and once at 17 weeks. Gavage was performed to maintain mice at low ascorbate values while preventing demise from scurvy (see supplementary methods). N = 5 mice per point. Samples from ascorbate unsupplemented mice indicated by (); samples from ascorbate supplemented mice indicated by (●). Individual data points are also included in Fig. 1B.
C. RBC osmotic fragility in Gulo−/− mice as a function of RBC ascorbate. 50% RBC lysis (see Fig. 2A) was tested in relation to RBC ascorbate concentration from 5 Gulo−/− mice supplemented with ascorbate 1 g/L for 17 weeks or 10 Gulo−/− mice deprived of ascorbate for 0–14 weeks, and then supplemented by gavage with 0.2 mg of ascorbate in 100 μL water once at 14 weeks and once at 17 weeks (see Fig. 2B and supplementary methods). N = 5 mice per point. Star symbols represent 50% lysis of RBCs from Gulo−/− mice unsupplemented (red) or supplemented (black) with ascorbate from Fig. 2A. Samples from ascorbate unsupplemented mice indicated by (); samples from ascorbate supplemented mice indicated by (●).
D. Biconcave and total diameters of RBCs from ascorbate supplemented and unsupplemented Gulo−/− mice. Ascorbate supplemented Gulo−/− mice (N = 6) received ascorbate 1 g/L for 12 weeks, and unsupplemented mice (N = 6) had no ascorbate for 12 weeks (plasma ascorbate concentrations were 66 ± 10 and 1 ± 1 μM respectively, see Fig. 2G). Forty to fifty RBCs in full view orientation using confocal microscopy were randomly selected from each group for measurement of diameters. ***: p < 0.0001 (t-test). See supplementary Fig. 1B for images.
E. β-Spectrin in lysates of mouse RBCs. RBC lysates from ascorbate supplemented, unsupplemented, and unsupplemented and then resupplemented Gulo−/− male mice were prepared as described in methods. Lysates were analyzed by Western blots using antibodies to α-spectrin, β-spectrin, ankyrin-1, protein 4.2 and β-actin. β-actin was used as loading control, and triplicates are displayed. Densitometry analyses are below each band. Left panel: Gulo−/− supplemented vs. unsupplemented mice. Right panel: Gulo−/− supplemented mice vs. gulo−/− mice that were unsupplemented and then resupplemented for 10 days (labeled Gulo ascorbate recovery). In lower panel, β-spectrin in mouse RBCs was normalized to β-actin for protein band signal intensities, using data from control, depleted and repleted Gulo−/− mice in two upper panels. Plasma ascorbate concentrations were: 66 ± 10 (supplemented mice, N = 6); 1 ± 1 μM (unsupplemented mice for 12 weeks, N = 6); and 61 ± 9 μM (unsupplemented mice for 12 weeks then resupplemented for 10 days, N = 6). Mice were resupplemented with ascorbate 2 g/L in drinking water to ensure rapid supplementation.
F. Osmotic RBC fragility as a function of NaCl concentration. Gulo−/− mice were deprived of ascorbate for 12 weeks (), and the same mice were supplemented with ascorbate 2 g/L in drinking water for the following 10 days (). N = 5 mice per point. The 50% lysis point for each condition (horizontal line) is determined by the vertical line of the same color. Plasma ascorbate concentrations (see supplementary Fig. 1C): Gulo−/− mice without ascorbate, 1.0 ± 1 μM; Gulo−/− mice supplemented with 2 g/L ascorbate for 10 days, 61 ± 9 μM. For unsupplemented vs. resupplemented RBC 50% lysis: p < 0.005 (two tailed t-test).
G. Ascorbate concentrations in plasma and RBCs from Gulo−/− male mice. Mice were continuously supplemented (N = 5); unsupplemented (N = 8); and unsupplemented then resupplemented (N = 6). Unsupplemented Gulo−/− mice did not receive ascorbate for 12 weeks. Resupplemented mice received ascorbate in drinking water 2 g/L for 10 days. Resupplemented mice had previously not had ascorbate for 12 weeks.
To get clues about osmotic fragility induced by ascorbate deficiency, we examined RBCs from ascorbate repleted and depleted Gulo−/− mice by confocal microscopy. A decreased surface-to-volume ratio indicates swelling, and internal diameters of RBCs are an inverse function of swelling because swelling produces a decreased surface-to- volume ratio (Diez-Silva et al., 2010). A decreased surface to volume ratio would be expected to decrease deformability, which was observed (Fig. 2D). Internal diameters (biconcave diameters) of RBCs were measured using confocal microscopy, and compared to controls, ascorbate-deficient RBCs had significantly lower internal diameters and a spherocyte-like appearance (Fig. 2D, supplementary Fig. 1B). The spherocyte appearance in these mouse RBCs was similar to RBCs from humans with mild hereditary spherocytosis, one of the few clinical disorders in which RBCs have increased osmotic sensitivity. Given the similarities in RBC appearance, and because human hereditary spherocytosis may be due to defects in spectrins or ankyrin or both, we used RBCs from Gulo−/− mice to quantitate these proteins by Western blot. Gulo−/− mice were ascorbate supplemented, ascorbate depleted, or ascorbate depleted followed by re-supplementation. Ankyrin-1 and α-spectrin were unchanged, but β-spectrin was significantly less in RBCs from depleted Gulo−/− male mice compared to controls (Fig. 2E left side). Depleted Gulo−/− male mice were repleted with ascorbate for 10 days, and RBCs were re-studied by Western blot (Fig. 2E right side); osmotic sensitivity (Fig. 2F); and confocal microscopy (supplementary Fig. 1C). β-Spectrin in RBCs from depleted then re-supplemented Gulo−/− mice was similar to β-spectrin in RBCs from continuously supplemented Gulo−/− mice (Fig. 2E right side). Western blot findings for β-spectrin are summarized in Fig. 2E (lower panel). Osmotic fragility measurements indicated that compared to controls, ascorbate depleted mice had increased fragility, which was reversed with ascorbate supplementation (Fig. 2F). RBC appearance in depleted then re-supplemented Gulo−/− mice was indistinguishable from controls (supplementary Fig. 1C). Ascorbate values in plasma and RBCs confirm supplementation, depletion, and repletion (Fig. 2G). Other than described above, RBCs from ascorbate supplemented and deficient male mice had indistinguishable hematologic parameters (supplementary Fig. 1D). Using female Gulo−/− mice, we evaluated ankyrin-1 and β-spectrin in ascorbate supplemented and unsupplemented mice; and in ascorbate depleted mice that were then re-supplemented for 3 days (supplementary Fig. 1E-G). Consistent with the findings using RBCs from male Gulo−/− mice, the data showed that only β-spectrin declined, that the decline reversed within as short a time as 3 days, and that ascorbate depletion and repletion values in plasma and RBCs were as predicted. Additionally, acute in vitro supplementation of RBCs from ascorbate deficient Gulo−/− mice did not reverse the decline in β-spectrin that was a consequence of low RBC ascorbate (supplementary Fig. 1H).
3.3. Potential Relationship to Diabetes: Dehydroascorbic Acid and Ascorbic Acid Transport into Human and Mouse RBCs
The potential connection between ascorbate, RBCs, and diabetes is that the probable species transported into RBCs is oxidized ascorbate, or dehydroascorbic acid (DHA), which enters via glucose transporters and then is immediately reduced intracellularly (Hughes and Maton, 1968, Mann and Newton, 1975, Bianchi and Rose, 1986, Mendiratta et al., 1998). To delineate the transported species in human and mouse RBCs, we used estimated physiologic plasma concentrations of DHA, which are 1–5% of ascorbate (Dhariwal et al., 1991, Lykkesfeldt, 2000); physiologic plasma ascorbate concentrations (Levine et al., 1996, Levine et al., 2001); freshly synthesized DHA (Rumsey et al., 1997, Corpe et al., 2013); and a recently developed assay for RBC ascorbate (Li et al., 2012). Extracellular DHA as substrate was measured to verify its presence (see Fig. 3 and supplementary Fig. 2). In human RBCs, DHA at 2 μM was avidly transported, such that within 2 min nearly all DHA moles in media were translocated into RBCs and reduced to ascorbate (Fig. 3A, supplementary Fig. 3 legend). No ascorbate was transported under the same conditions (Fig. 3A). Using DHA and ascorbate concentrations of 5 μM (supplementary Fig. 3A,) and human RBCs, similar findings were obtained: only DHA was transported. Mouse RBCs also transported only DHA and not ascorbate, but transport rates were 10–20 fold lower compared to human RBCs (Fig. 3B; supplementary Fig. 3B). Only ascorbate was found in RBCs (Li et al., 2012) (supplementary Fig. 4A,B).
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Fig. 3. Dehydroascorbic acid and ascorbic acid transport into human and mouse RBCs as a function of time.
Humans RBCs are in grouped Figure A, mouse RBCs are in Figure B. Each grouped figure contains four panels. For each grouped figure: in the left upper panel, RBC ascorbate concentration is shown after incubation with one concentration of dehydroascorbic acid; and in the right upper panel, RBC ascorbate concentration is shown after incubation with the same concentration of ascorbic acid. The lower panels are the corresponding extracellular concentrations: dehydroascorbic acid added on the left lower side, ascorbic acid added on the right lower side. Within each panel, a different symbol represents blood from a different human or mouse, and from each human or mouse three or more independent samples were obtained and analyzed at every time point. All open black and white symbols on both sides indicate sample analyses by HPLC, with no reducing agent added. For color symbols: green symbols (, ) indicate analyses by HPLC, with reducing agent (500 μM TCEP) present during incubation; red symbols (, ) indicate analyses by scintillation spectrometry, without reducing agent added; blue symbols (, ) indicate analyses by scintillation spectrometry, with reducing agent (500 μM TCEP) present during incubation.
The concentration of dehydroascorbic acid/ascorbic acid was 2 μM. For clarity, concentrations and species are shown in each panel. Additional experiments with concentration of 5 μM is shown in supplementary figures. For human RBC experiments, 50 μL RBCs were incubated in 0.5 mL total volume using PBS with 5 mM glucose. For mouse RBC experiments, 30 μL RBCs were incubated in 0.3 mL total volume using PBS with 5 mM glucose. Note that initial concentrations are different in the same figures because animals/human often do not have the same initial values, and some data represent scintillation spectrometry results, where initial values should be close to or at zero. See methods and supplementary Fig. 3 legend for other details.
3.4. Glucose Inhibition of DHA Uptake into RBCs in vitro, GLUT Transporters Expressed, and Glucose Effects on RBC Ascorbate Concentrations and Osmotic Fragility in vivo
Since DHA is exclusively transported, and the likely transporter is a facilitated glucose transporter (GLUT 1, Glut 3) (Vera et al., 1993, Rumsey et al., 1997, Corpe et al., 2013), we investigated DHA transport inhibition by the non-metabolized analog 3-O-methylglucose, using human RBCs. As essential control, [3H]3-O-methylglucose 5 μM uptake was inhibited by increasing concentrations of unlabeled 3-O-methylglucose 1–30 mM (Fig. 4A; supplementary Fig. 5A-E). Under the same conditions, 5 μM [14C]DHA uptake was inhibited by 1–30 mM 3-O-methylglucose in a matching pattern (Fig. 4B). DHA was fully reduced to ascorbate under these experimental conditions (supplementary Fig. 6). To account for transactivation and to maximize transport, RBCs were pre-loaded with 3-O-methylglucose (Stein, 1986, Carruthers and Naftalin, 2009, Carruthers et al., 2009, Sage and Carruthers, 2014). The glucose transporter inhibitor, cytochalasin B, inhibited uptake of 3-O-methylglucose and DHA nearly identically in human RBCs (Fig. 4C,D). With mouse RBCs, 30 mM unlabeled 3-O-methylglucose inhibited [3H]3-O-methylglucose and DHA uptake, although when compared to human RBCs, inhibition of DHA uptake by 3-O-MG was less robust (Fig. 4E,F). Similar findings in mouse RBCs were obtained with cytochalasin B as an inhibitor of [3H]3-O-methylglucose and DHA uptake: when compared to human RBCS, inhibition of DHA uptake by cytochalasin B was less robust (Fig. 4G,H). Based on Fig. 4, human RBCs transported DHA and 3-O-MG similarly, while mouse RBCs had a higher rate of 3-O-MG transport than DHA. Slower transport of DHA in mouse RBCs compared to human RBCs is consistent with the data in Fig. 3.
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Fig. 4. Glucose Inhibition of DHA uptake into RBCs in vitro; glucose effects on ascorbate concentrations in RBCs in vivo.
A, B. Inhibition of 3-O-[3H]MG and [14C]DHA uptake into human RBCs by unlabeled 3-O-MG. After loading with 20 mM 3-O-MG at 37 °C, human RBCs 10% were washed and incubated on ice for 1 min with 0–30 mM unlabeled 3-O-MG as indicated and with either 5 μM 3-O-[3H]MG as control (A) or 5 μM [14C]DHA (B). Uptake of 3-O-MG and DHA were determined by scintillation spectrometry.
C,D. Inhibition of 3-O-[3H]MG and [14C]DHA uptake into human RBCs by cytochalasin B (CytB). After loading with 20 mM 3-O-MG at 37 °C and without washing, human RBCs 10% were incubated on ice for 1 min with (■) or without (●) 20 μM cytochalasin B and with 5 μM 3-O-[3H]MG (C) or with 5 μM [14C]DHA (D).
E,F. Inhibition of DHA uptake into WT mouse RBCs by 3-O-MG. After loading with 20 mM 3-O-MG, mouse RBCs 10% were washed and incubated at 37 °C for 1 min. For control experiments (E), 5 μM 3-O-[3H]MG was added alone or with 30 mM 3-O-MG. For DHA uptake experiments (F), 5 μM [14C]DHA was added alone or with 30 mM 3-O-MG.
G,H. Inhibition of DHA uptake into WT mouse RBCs by cytochalasin B. After loading with 20 mM 3-O-MG, mouse RBCs 10% were washed and incubated at 37 °C for 1 min. For control experiments (G), 5 μM 3-O-[3H]MG was added alone or with 20 μM cytochalasin B. For DHA uptake experiments (H), 5 μM [14C]DHA was added alone or with 20 μM cytochalasin B.
Because transport of DHA and its inhibition were less robust in mouse RBCs in vitro compared to human RBCs in vitro, it was uncertain whether glucose would affect RBC ascorbate concentrations in mouse RBCs in vivo. To investigate, we used the AZIP lipoatrophic diabetes mouse model (Moitra et al., 1998). These mice have stable hyperglycemia that reverses overnight with fasting. RBC ascorbate and blood glucose, measured in non-fasted and fasted AZIP and WT littermate-control mice, displayed an inverse relationship (Fig. 5A). The decrease in RBC ascorbate induced by hyperglycemia occurred between 100 and 200 mg/dl glucose (5.5–11.1 mM), a key range in diabetes (American Diabetes Association: Position Statement, 2014, American Diabetes Association: Position Statement, 2015). To learn whether changes in ascorbate plasma values could explain the inverse relationship between plasma glucose and RBC ascorbate, plasma ascorbate values were measured as controls in all groups (Fig. 5B). The findings showed that there were no statistically significant changes in plasma ascorbate concentrations in normal and AZIP mice as a function of plasma glucose concentrations. Therefore, plasma ascorbate concentrations did not account for the observed relationship between glucose and RBC ascorbate. As a corollary, we investigated whether ascorbate affected glucose values in ascorbate supplemented and depleted Gulo−/− mice. The values were not statistically different: 162 ± 14 mg/dl (9 ± 0.7 mM) vs 168 ± 21 mg/dl (9.3 ± 1.2 mM), respectively, N = 6 per group.
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Fig. 5. RBC and plasma ascorbate in mice in relation to glycemia.
A. RBC ascorbate as a function of blood glucose in fed and fasted control (, ) and fed and fasted diabetic AZIP mice (, ). AZIP mice and control littermates were fed chow ad libitum until 16 h before sacrifice. Blood was withdrawn from mice that were fed or fasted 16 h and RBC ascorbate measured by HPLC. AZIP mice were tested together with littermate controls.
B. Ascorbate plasma concentrations as a function of blood glucose in fed () or fasted AZIP () mice and fed () or fasted () WT controls. Control and AZIP mice synthesize ascorbate and do not have an intake requirement for it. There was no significant difference between plasma ascorbate values in fasted AZIP mice compared to fasted WT control mice.
Because DHA transport rates are 10–20 fold different in mouse and human RBCs, and given conflicting data for transporter activities (Montel-Hagen et al., 2008b, Sage and Carruthers, 2014), we characterized GLUTs in these cells (Fig. 6A-G). Human RBCs expressed GLUT 1 dominantly, with trace expression of GLUTs 2–4, generally consistent with prior findings (Concha et al., 1997). Mouse RBCs expressed Gluts 3 and 4 but not Glut 1, even when a high sensitivity method was used (Fig. 6E). Transporter activity data indicate that GLUTs 1 and 3 are by far the most robust DHA transporters (Rumsey et al., 1997, Rumsey et al., 2000, Corpe et al., 2013). Using Xenopus oocytes, we show here that human GLUT 1 and mouse Glut 3 transported DHA similarly (Fig. 6G). Considered together, the data suggest that GLUT 1 is the dominant DHA transporter in human RBCs, while mouse RBCs utilize Glut 3, and perhaps Glut 4. Although mouse Glut 3 and human GLUT 1 had similar transport characteristics, there was substantially less Glut 3 in mouse RBCs compared to GLUT 1 in human RBCs (Fig. 6A, C). These findings are consistent with slower DHA transport in mouse RBCs compared to human RBCs (see Fig. 3, Fig. 4).
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Fig. 6. GLUTs in mouse and human RBCs and relevant transport activities.
A-F. GLUTs in mouse and human RBCs as determined by Western blot.
For GLUT 1 analyses, 2.5 μL of human or mouse RBC lysate (RBC 5% v/v) and 20 μg of NIH/3 T3 cell lysate were analyzed. For mouse Glut 1 and Glut 2, 3 and 4 analyses: 10 μL of lysate were analyzed from RBCs (RBC 20% v/v), cells (NIH/3 T3, Caco-2, U87-MG or MCF-7) or tissues (mouse intestine, brain or skeletal muscle). β-actin was used as loading control in each blot, and α/β spectrin were used to indicate RBC membrane separation.
RBC ghosts were prepared as described (Steck and Kant, 1974).
G. DHA uptake in injected Xenopus laevis oocytes. Mouse Glut 3 and human GLUT 1 cRNAs were injected and expressed in Xenopus laevis oocytes, as described (Rumsey et al., 1997). cRNA concentrations were 1 ng/nL. Sham-injected oocytes were injected with 36.8 nL of water. Each point is mean value of 10 oocytes ± S.D.
Due to these distinct GLUT transporter differences on RBCs from mice and humans, we characterized human RBCs from healthy and diabetic subjects with respect to ascorbate, osmotic fragility, and β-spectrin. Ascorbate in RBCs from these subjects was inversely correlated with fasting glucose (Fig. 7A, supplementary Fig. 7); and osmotic fragility and severity of diabetes (Fig. 7B). For osmotic fragility, p < 0.05 for healthy subjects vs. poorly controlled diabetic subjects (hemoglobin A1C > 7.8). The clinical data describing osmotic fragility in relation to ascorbate concentration are consistent with mouse findings (Fig. 2). Similarly, the clinical data indicating that hyperglycemia is associated with lower RBC ascorbate concentrations are consistent with mouse findings (Fig. 5). Compared to healthy subjects, β-spectrin progressively declined in subjects with mild and poorly controlled diabetes, while α-spectrin was unchanged (Fig. 7C, D, E). RBC ascorbate values are shown for these subject groups in Fig. 7E. A linear relationship existed between plasma and RBC ascorbate with hyperglycemia as well as with euglycemia (supplementary Fig. 7). With data displayed in this fashion, it was recapitulated that RBC ascorbate was significantly lower with hyperglycemia compared to euglycemia (supplementary Fig. 7). How hyperglycemia dynamically affects RBC ascorbate will be best determined in the same diabetic patients with and without hyperglycemia in future clinical experiments.
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Fig. 7. RBC ascorbate and β-spectrin in healthy and diabetic humans.
A. Human RBC ascorbate concentrations as a function of plasma glucose concentrations. N = 39 and R = − 0.46.
B. Osmotic fragility in human RBCs as a function of RBC ascorbate concentrations. Symbols as follows: (), healthy subjects hemoglobin A1C 4.5–5.6; (), diabetic subjects hemoglobin A1C (HgbA1C) 5.7–7.7; (), diabetic subjects hemoglobin A1C 7.8–10.6. Statistics for the line describing all data: N = 42 and R = − 0.41. Differences between () healthy subjects vs () diabetic subjects were statistically significant, p < 0.05.
C, D, E. Human RBC α-spectrin, β-spectrin, and β-actin in healthy subjects (14); subjects with mild diabetes (13); and subjects with poorly controlled diabetes (13). Normalized values are in D and subject profiles in E. Subjects were randomly selected based on hemoglobin A1C and sample availability.
4. Discussion
The data presented here provide original links between red blood cell osmotic fragility, vitamin C, and diabetes. Hemolysis was observed when whole blood was centrifuged from mice with low vitamin C plasma and RBC concentrations. Similar low vitamin C concentrations were found in some human subjects from random sampling, and were consistently produced in mice unable to synthesize vitamin C. Mouse RBC hemolysis induced by low RBC ascorbate concentrations was quantitated by osmotic fragility, and reversed when mice were repleted with vitamin C. Mouse RBCs with increased fragility induced by low RBC ascorbate were spherocyte-like, with decreased β-spectrin. With repletion of RBC ascorbate, osmotic fragility and spherocyte appearance reversed, and β-spectrin was restored. Entry of vitamin C into RBCs was only via the oxidized form dehydroascorbic acid: ascorbic acid itself was not transported. Because dehydroascorbic acid and glucose have similar affinities for facilitated glucose transporters in vitro, our findings suggested an innovative connection between dehydroascorbic acid and diabetes. The putative link was strengthened by experiments using mouse and human RBCs. Advantages of studies here with dehydroascorbic acid are that dehydroascorbic acid was synthesized de novo and its disappearance accounted for. Dehydroascorbic acid transport, performed for the first time approximating physiologic concentrations, was progressively inhibited by glucose concentrations found in diabetes (Fig. 4A and B). Glucose concentrations in vivo, from diabetic mice and WT controls, were inversely associated with RBC ascorbate concentrations. Despite differences in GLUT identities in mouse versus human RBCs, human RBC ascorbate concentrations were again inversely related to plasma glucose, osmotic fragility, and severity of diabetes. β-spectrin progressively declined in RBCs as diabetes worsened.
Considered together, these unique findings support several parts of an original overarching hypothesis, famine from feast (Fig. 8), and provide innovative mechanism links between vitamin C and diabetes. In essence, diabetes pathophysiology might produce ascorbate deficiency in RBCs, with potential vascular pathophysiologic consequences. Increased RBC rigidity from low ascorbate would cause microvascular, or local, hypoxia by slowing RBC capillary flow, thereby decreasing oxygen delivery per unit time. The exciting therapeutic aspect is that local deficiency might be reversible with oral ascorbic acid or dehydroascorbic acid or both, or intravenous ascorbic acid (Lamas et al., 2013).
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Fig. 8. Famine From Feast Hypothesis.
In both mice and humans, data indicated that ascorbate concentrations in RBCs were inversely related to hyperglycemia. Competition in vivo between DHA and glucose is an attractive explanation, but there are multiple paths that could lead to low RBC ascorbate in diabetes. For RBCs, diabetes in humans could result in: downregulation of GLUT1 transporter expression; change in GLUT transporter type expressed; reduced avidity of GLUT 1 transporters for DHA, i.e. from oxidative damage (Nishikawa et al., 2000, Yu et al., 2006, Wang et al., 2012); competition between DHA and elevated glucose concentrations; accelerated ascorbate utilization coupled to sorbitol formation within RBCs or exogenous oxidant generation from hyperglycemia (Nishikawa et al., 2000, Yu et al., 2006, Wang et al., 2012); or altered vitamin C – vitamin E recycling. Beyond RBCs, plasma ascorbate may be lower in diabetes due to: less ascorbate intake; decreased ascorbate intestinal absorption; increased glomerular filtration rate (GFR) or SVCT1 impairment leading to diminished reabsorption; or increased ascorbate utilization. If for any of these reasons plasma vitamin C concentrations were lower, less dehydroascorbic acid would then be available for RBC entry.
Anemia is common in diabetes, with multiple causes (Thomas et al., 2003, Thomas et al., 2006, Craig et al., 2005). Low RBC ascorbate might contribute to anemia, possibly via low grade chronic hemolysis produced by RBC rigidity. Future studies of diabetic subjects will include measurement of cell-free hemoglobin and reticulocyte counts in relation to ascorbate. Future studies will also address whether increased RBC osmotic fragility caused by low ascorbate has consequences to hemoglobin function.
Consistent with the famine from feast hypothesis (Fig. 8), it has been reported that low vitamin C plasma concentrations occur in diabetes (Will and Byers, 1996, Chen et al., 2006). However, prior associations between vitamin C and diabetes did not account mechanistically for vascular complications, other than by generalized oxidative damage. Except for the dataset presented here, there has been no recognition that humans with diabetes have lower than expected vitamin C concentrations in their RBCs. Unfortunately, most prior existing datasets for plasma vitamin C concentrations in diabetic subjects are confounded by assay artifacts; by prior inabilities to measure RBC ascorbate; and by inabilities to measure dehydroascorbic acid directly in plasma or RBCs (Will and Byers, 1996, Li et al., 2012, Dhariwal et al., 1991, Lykkesfeldt, 2000). Surprisingly, modern hematology textbooks do not even describe that there is vitamin C in RBCs (Kaushansky et al., 2010, Greer et al., 2013). To our knowledge, the data here displaying RBC vs plasma vitamin C concentrations are the most comprehensive available to date in healthy and diabetic humans.
Previous investigators found no link between dehydroascorbic acid transport and glucose in mouse and human RBCs (Montel-Hagen et al., 2008a), but the findings may have been due to use of radiolabel without any direct ascorbate measurements; dehydroascorbic acid degradation (Rumsey et al., 1997); flawed kinetics assumptions (Rumsey et al., 1997, Carruthers and Naftalin, 2009); metabolism of glucose and the analog 2-deoxyglucose (Carruthers et al., 2009); and lack of accounting for GLUT transactivation (Cloherty et al., 1996). Based on findings on human RBCs, high co-expression of GLUT 1 with stomatin was interpreted to enhance DHA transport but negatively modulate glucose uptake specifically in human RBCs (Montel-Hagen et al., 2008b). Recent data using RBCs and inside out vesicles do not support a role for a stomatin-regulated pool of GLUT 1 that preferentially transports DHA rather than glucose. Instead, DHA and glucose were transported similarly by GLUT 1 (Rumsey et al., 1997, Sage and Carruthers, 2014). The presence of Glut 4 in mouse RBCs was previously reported (Montel-Hagen et al., 2008b), and confirmed in the results here. While Glut-dependent DHA uptake was concluded to be absent from mature murine RBCs (Montel-Hagen et al., 2008b), the findings in the present manuscript suggest this conclusion is incorrect.
In the current experiments we have not assessed dehydroascorbic acid transport activity of all 14 identified GLUTs (Mueckler and Thorens, 2013). In previous experiments, Xenopus oocytes were microinjected with cRNAs for GLUTs 1–12 to assess DHA transport activity. GLUTs 1 and 3 were at least 10 fold more active than other transporters (Rumsey et al., 1997, Rumsey et al., 2000, Corpe et al., 2013). Data here are consistent with these reports. There are no data available describing DHA transport by GLUTs 13 and 14. GLUT 13 is expressed predominantly in brain, and transports myo-inositol but not glucose. Although GLUT 14 may have arisen as a gene duplication of GLUT 3, there is no rodent homolog, and GLUT 14 is expressed mainly in testis. Relying on identities of GLUTs in human RBCs, GLUT transport activity for DHA, and known distribution of GLUTs 1–14, we and others conclude that in human RBCs DHA is transported by GLUT 1 (Montel-Hagen et al., 2008b, Sage and Carruthers, 2014). Based on similar reasons, we conclude that mouse RBCs utilize Glut 3 and Glut 4 for DHA transport, but it is unclear which transporter predominates.
DHA uptake is unlikely to be due to simple diffusion for several reasons (see Fig. 4, Fig. 6). Unlabeled 3-O-methylglucose inhibited [3H]3-O-methylglucose uptake and [14C]DHA uptake similarly. Likewise, cytochalasin B inhibited [14C]DHA uptake and [3H]3-O-methylglucose uptake in a comparable manner. In Xenopus oocytes, DHA transport occurred only when GLUTs were injected. There was no uptake with sham injection. Together, these data support the conclusion that DHA is transported on glucose transporters. While DHA diffusion cannot be excluded in mammalian cells, glucose as a similar molecule requires a membrane transporter and is transported approximately 104 faster than diffusion (Elbrink and Bihler, 1975, Mueckler et al., 1985).
We show here that RBC β-spectrin decreased when RBC ascorbate concentrations were low. With ascorbate repletion, decreased β-spectrin was restored over as short a time as three days. The average life span of mouse RBCs is ~ 40 days (Van Putten, 1958). Thus, β-spectrin recovery is not due to appearance of new RBCs. We speculate that changes in β-spectrin may be due to reversible oxidative modification that is specific for ascorbate as an electron donor in RBCs. Although the full β-spectrin crystal structure is not available, no disulfide bonds were detected in the available crystal structure, suggesting that potential oxidative modification(s) occur at other sites (Ipsaro et al., 2010). Future experiments will explore these possibilities. Hemoglobin and spectrin can form complexes that may be facilitated by oxidation. In one report, complexes formed upon hydrogen peroxide treatment in vitro (Snyder et al., 1985), but only 0–5.9% of spectrin-hemoglobin was cross-linked by treating with 45–810 μM H2O2. In another report, there were only trace amounts of a spectrin-hemoglobin complex which was detected on Western blot using antibody against hemoglobin (Fortier et al., 1988). In our studies, we did not observe any shifted spectrin bands using either antibody against both α and β spectrins or specific β-spectrin antibody, suggesting that a spectrin-hemoglobin complex is below detection limits. The existence of other ascorbate- specific effects is a reasonable explanation of the decreased β-spectrin levels that we observed in ascorbate depleted Gulo−/− mice and poorly controlled diabetic subjects.
Experiments here with ascorbate in mouse RBCs provide original insights coupling RBC fragility to low ascorbate concentrations. Notably, humans have an essential requirement for ascorbate; human RBCs have far more avidity than mouse RBCs for DHA; and human and mouse RBCs have distinctly different GLUT transporter expression. These species differences matter, and therefore human rather than rodent experimentation is now the way forward. Clear clinical research possibilities exist to study RBCs in humans with diabetes in relation to ascorbate. Many possibilities are described above. Additional experimental goals in humans are to characterize RBC ascorbate concentrations dynamically in humans with diabetes, as a function of glycemia in each subject. To do so, diabetic subjects with low ascorbate can be hospitalized; their RBC deformability parameters can be measured before and during controlled hyperglycemia; after discharge, these subjects can be supplemented with ascorbate to increase their RBC ascorbate concentrations; post supplementation, subjects can be re-hospitalized to again measure RBC properties with controlled hyperglycemia. Only after RBC ascorbate concentrations are characterized dynamically in diabetic subjects can longer term goals be approached. These include determination of oxygen delivery to a relevant appropriate microvascular circulation, and/or clinical outcomes (i.e. progression of retinopathy) as a function of RBC ascorbate concentrations. Although it will take time to address unknowns through clinical experimentation, the experimental outcomes have the possibility to substantially impact clinical practice, and initial studies have begun (NCT02107976 and NCT00071526, clinical trials.gov).https://www.sciencedirect.com/science/article/pii/S235239641530164X
Third, supplementation with vitamin C improved endothelial dysfunction in apoE-deficient mice, reduced atherosclerotic lesions, and restored eNOS enzymatic activity. This is most likely due, in part, to the ability of vitamin C to protect BH 4 and to preserve biosynthesis of NO.Author: Livius V. d’Uscio, Sheldon Milstien, Darcy Richardson, Leslie Smith, Zvonimir S. KatusicCited by: 331Publish Year: 2003www.ahajournals.org/doi/full/10.1161/01.res.0000049166.33035.62
