芦丁(Rutin)通过激活AMPK,增加肌肉中的线粒体合成

Rutin Increases Muscle Mitochondrial Biogenesis with AMPK Activation in High-Fat Diet-Induced Obese Rats

 

研究机构:

韩国首尔梨花女子大学营养科学与食品管理系

高丽大学食品与营养系,韩国首尔大学

 

 

摘要

 

线粒体数量减少和骨骼肌功能紊乱与肥胖和肥胖相关的代谢紊乱有关。本研究的具体目的是研究芦丁对高脂肪饮食诱导肥胖大鼠骨骼肌线粒体生物发生的影响。

 

补充芦丁可以降低体重和脂肪组织质量,尽管能量摄入相等(p < 0.05)。芦丁显著增加线粒体大小和线粒体DNA(mtDNA)内容以及基因表达与线粒体生物起源有关,如过氧化物酶体增殖物激活受体γ辅激活子1α, 核呼吸因子- 1(NRF-1)转录因子(Tfam),和烟酰胺腺嘌呤二核苷酸(NAD)端依赖脱乙酰酶,s irtulin1 SIRT1在骨骼肌(p < 0.05)

 

此外,芦丁的摄入增加了肌腺苷单磷酸酶(AMPK)活性40% (p < 0.05)

 

综上所述,这些结果表明,肌线粒体和AMPK激活至少部分参与了芦丁(rutin)介导的对肥胖症有益的作用。

 

常见的食物如苹果(皮)、苦荞麦、芸香叶、烟叶、枣、杏、橙皮、番茄、绿茶含丰富的芦丁(Rutin)。

 

Rutin Increases Muscle Mitochondrial Biogenesis with AMPK Activation in High-Fat Diet-Induced Obese Rats

 

Sangjin Seo 1,†, Mak-Soon Lee 1,†, Eugene Chang 1,†, Yoonjin Shin 1, Soojung Oh 1, In-Hwan Kim 2 and Yangha Kim 1,*  OrcID

1 Department of Nutritional Science and Food Management, Ewha Womans University, Seoul 120-750, Korea

2 Department of Food and Nutrition, Korea University, Seoul 136-703, Korea

 

Abstract

Decreased mitochondrial number and dysfunction in skeletal muscle are associated with obesity and the progression of obesity-associated metabolic disorders. The specific aim of the current study was to investigate the effects of rutin on mitochondrial biogenesis in skeletal muscle of high-fat diet-induced obese rats. Supplementation with rutin reduced body weight and adipose tissue mass, despite equivalent energy intake (p < 0.05). Rutin significantly increased mitochondrial size and mitochondrial DNA (mtDNA) content as well as gene expression related to mitochondrial biogenesis, such as peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α), nuclear respiratory factor-1 (NRF-1), transcription factor A (Tfam), and nicotinamide adenine dinucleotide (NAD)-dependent deacetylase, sirtulin1 (SIRT1) in skeletal muscle (p < 0.05). Moreover, rutin consumption increased muscle adenosine monophosphate-activated protein kinase (AMPK) activity by 40% (p < 0.05). Taken together, these results suggested at least partial involvement of muscle mitochondria and AMPK activation in the rutin-mediated beneficial effect on obesity.

 

http://www.mdpi.com/2072-6643/7/9/5385