生姜杀死癌细胞的机理 诱导产生活性氧,触发 皮肤癌细胞的自杀

[6]-姜酚诱导活性氧在人表皮样癌A431细胞中调控线粒体细胞死亡途径。

[6]-Gingerol induces reactive oxygen species regulated mitochondrial cell death pathway in human epidermoid carcinoma A431 cells

 

翻译:蓝山

 

研究机构:印度毒物学研究所蛋白质组学实验室(CSIR) P.O. Box 80, m.g Marg, Lucknow 226001,印度。

 

由于皮肤癌的发病率和患病率不断上升,尽管所有的努力,寻找新的,更好的保护和治疗的代理是必需的。生姜是一种单子叶植物,被广泛应用于中草药,因为它含有相应的酚酮,其中[6]-姜酚是主要的一种。‘

 

 

在印度市场常见的可用生姜品种总,鲜姜中的姜辣素的含量在粉末在104 - 965μg / g。在此,[6]-姜酚对人皮肤癌A431细胞的抗凋亡作用进行了评估。[6]-姜酚的治疗表现出了相当大的细胞毒性,其表现为通过产生活性氧(ROS)A431细胞生长抑制。ROS的增加导致线粒体膜电位(MMP)的减少和细胞凋亡的诱导。

 

结果显示,线粒体膜的干扰与基因转录水平和蛋白水平的Bax/Bcl-2比值的解除有关,而[6]-姜酚的治疗导致细胞色素-Capaf1的上调,最终导致Caspase级联的触发。这表明[6]-姜酚可以有效地用于治疗皮肤癌。

 

 

参看文献:

 

https://www.sciencedirect.com/science/article/pii/S0009279709001859

 

[6]-Gingerol induces reactive oxygen species regulated mitochondrial cell death pathway in human epidermoid carcinoma A431 cells

 

Research organization: Proteomics Laboratory, Indian Institute of Toxicology Research (CSIR), P.O. Box 80, M.G. Marg, Lucknow 226001, India

 

Since skin cancer incidence and prevalence is constantly rising up the charts despite all efforts, search for newer, better agents for protection and treatment is required. Ginger (Zingiber officinale Roscoe), a monocotyledonous herb, is widely used as a herbal medicine, given the presence of homologous phenolic ketones, of which [6]-gingerol is the major one. The quantity of [6]-gingerol in the fresh ginger rhizome was found to be 104–965 μg/g in common varieties of ginger available in Indian market. Herein, [6]-gingerol was assessed for its anti-apoptotic effects in human epidermoid carcinoma A431 cells. [6]-Gingerol treatment exhibited considerable cytotoxicity as indicated by growth inhibition of A431 cells mediated via generation of reactive oxygen species (ROS). Increase in ROS led to decrease in mitochondrial membrane potential (MMP) and subsequent induction of apoptosis. Results revealed that perturbations in mitochondrial membrane are associated with deregulation of Bax/Bcl-2 ratio at gene transcriptional level as well as protein level, where treatment with [6]-gingerol leads to up-regulation of Cytochrome-c and Apaf-1 subsequently culminating in triggering of Caspase cascade. These firmly suggest that [6]-gingerol can be effectively used for the treatment of skin cancer.

 

https://www.sciencedirect.com/science/article/pii/S0009279709001859