Ginger Kills Leukemia Cells, Found by China Pharmaceutical University
在这个发表在《分子肿瘤Molecular Cancer》（Molecular Cancer是一份开放访问，同行评审的在线杂志）上的研究中，研究人员发现，姜烯酚（6-Shogaol）在体外和体内都能诱导人白血病细胞凋亡(细胞死亡)，而没有副作用!
6-Shogaol induces apoptosis in transformed and primary leukemia cells. Cells apoptosis was determined using Annexin V/PI staining by flow cytometry. The values obtained from Annexin V/PI represent the mean±SD for three separate experiments. The difference were significant at *p < 0.05, **p < 0.01. (a) Jurkat cells were treated without or with various concentrations of 6-shogaol (6S) for 24 h. (b) Jurkat cells were treated without or with 15 μM for different time intervals as indicated. (c) Jurkat cell were treated without or with 6S for various concentrations or time intervals as indicated, total cellular extracts were prepared and subjected to western blot assay using antibodies against PARP, cleaved-caspase 3 (C-Caspase 3) and cleaved-caspase 7 (C-Caspase 7). (d and e) Jurkat, U937, and HL-60 cells were treated without or with 15 μM for 24 h, after which apoptosis was determined by flow cytometry. Total cellular extracts of protein were prepared and subjected to western blot using antibodies as indicated. (f) Mononuclear cells were isolated from the peripheral blood of 7 patients with leukemia (designated as P1–7), including 4 AML, 1 MM (P7), and 2 CLL patients (P3 and P4). Cells were then treated without or with 10 and 20 μM 6S for 24 hours. (g) Mononuclear cells were isolated from the peripheral blood of 5 healthy donors and incubated with 0, 10 and 20 μM of 6S for 24 hours.
6-Shogaol induces apoptosis in human leukemia cells through a process involving caspase-mediated cleavage of eIF2α
Qun Liu†, Yong-Bo Peng†, Ping Zhou, Lian-Wen Qi, Mu Zhang, Ning GaoEmail author, E-Hu LiuEmail author and Ping Li
State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, China