Yale University:Cold Viruses Attack When Your Immune System Is Cold
去年，由耶鲁大学的Ellen Foxman和Akiko Iwasaki领导的一个研究小组为一个流行的观点提供了有力的证据，那就是在寒冷的天气里一个人更容易感冒。他们发现，当小鼠在低于正常体温的温度下孵育时，它们的细胞对引起普通感冒的鼻病毒反应迟钝。
图的左侧描述了使用未感染细胞的控制条件。(“模拟”感染是该行业使用的术语。)然后对细胞进行染色，观察是否存在双链RNA (dsRNA，表示病毒感染)和蛋白casp3 (casp3+，表示细胞凋亡已被激活)。如左图所示，染色模式显示细胞既没有被感染也没有发生凋亡。这是意料之中的，因为这些框表示控制条件。
Cold Viruses Attack When Your Immune System Is Cold
Last year, a team of researchers led by Ellen Foxman and Akiko Iwasaki of Yale University provided supporting evidence for the popular notion that being in cold weather makes a person more susceptible to catching cold. They showed that mouse cells do not respond well to rhinovirus, a cause of the common cold, when incubated at temperatures lower than normal body temperature.
In particular, mouse cells secreted less and were less responsive to interferon-β, a type of antiviral defense molecule. Thus, their research demonstrated a plausible molecular mechanism to explain the phenomenon. Now, the researchers are back with yet more evidence. This time, they examined human cells and focused on immune processes that were not related to interferon-β. Intentional suicide (i.e., apoptosis) is one way that cells handle viral infection. Though this process results in the death of the cell, it helps prevent the spread of the virus to other cells. But, in one set of experiments, the team showed that infected cells were much less likely to commit suicide if they were incubated at 33 degrees C., which is four degrees lower than normal body temperature. (See figure.) Apoptosis triggered by viral infection is slowed at 33 degrees C.Apoptosis triggered by viral infection is slowed at 33 degrees C. The left-hand side of the figure depicts the control condition using cells that were not infected. ("Mock" infection is the term used in the trade.) Cells were then stained for the presence of double-stranded RNA (dsRNA, which indicates viral infection) and the protein caspase-3 (casp3+, which indicates that apoptosis has been activated). As depicted in both left-hand boxes, the staining patterns showed that cells were neither infected nor undergoing apoptosis. That is to be expected, since these boxes represent control conditions. The right-hand side of the figure portrays the experimental conditions in which cells were infected by rhinovirus. Notice that while both boxes show the presence of dsRNA, indicating viral infection, only a fraction of cells are undergoing apoptosis at 33 degrees compared to 37 degrees. (See the percentages circled in red.) The result is clear: A colder temperature slows down an infected cell's ability to commit suicide. A molecular mechanism that keeps viral infections in check.A molecular mechanism that keeps viral infections in check. Further experiments showed that when researchers suppressed apoptosis and RNaseL, a cellular enzyme that destroys dsRNA, rhinovirus proliferated even inside cells that were grown at 37 degrees C. This conclusively demonstrated the importance of these mechanisms for keeping viral infections in check. With their data, the authors constructed a model of what they believe is occurring inside an infected cell. Detection of dsRNA alerts the cell to the presence of a virus. The cell then activates apoptosis and RNaseL. In normal conditions, this may help prevent the spread of the virus. But at cooler temperatures (such as those found in our airways), these mechanisms don't work very well, leaving us susceptible to rhinovirus infection. So, when winter comes, be sure to wear a scarf around your nose and mouth... and begrudgingly admit that mom was right. Update (5:21 pm PT, July 11, 2016) Dr. Iwasaki kindly provided a figure that beautifully summarizes his team's model. Courtesy of Dr. Akiko Iwasaki.Courtesy of Dr. Akiko Iwasaki. ### Source: Ellen F. Foxman, James A. Storer, Kiran Vanaja, Andre Levchenko, and Akiko Iwasaki. "Two interferon-independent double-stranded RNA- induced host defense strategies suppress the common cold virus at warm temperature." PNAS. Published online: 11-July-2016. DOI: 10.1073/pnas.1601942113 Cold Viruses Attack When Your Immune System Is Cold | American Council on Science and Health https://www.acsh.org/news/2016/07/11/cold-viruses-attack-when-your-immune-system-is-cold